FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604860
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.039.785531
GGTATCA5350.039.394131
CGAATTA400.002591464234.51058615
GCTATAC501.8588331E-434.504883
TATCAAC13300.034.2681122
ATCAACG13350.034.1171843
TCAACGC13400.033.981454
CAACGCA13650.033.61185
AACGCAG14250.032.1965686
TTGTACC652.3214085E-531.8427564
ACGCAGA14850.031.127997
AGAGTAC15200.029.7328411
CAGAGTA15950.028.5510410
CGCAGAG16300.027.937988
GTGTACG500.00767696727.6404721
TTTATAC756.152829E-527.6039033
ACGGTAC500.007726733627.6039033
GCAGAGT16550.027.5159579
TAATCGT1057.505005E-726.35262938
GTGGTAT2100.026.324261
Produced by FastQC (version 0.11.5)