FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074234
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34150.31790094150808856No Hit
GTGTGTACCTTGCATGCAGGAATAAACCCCAACATCCTCAGCCTCCACCC32180.2995622927593057No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCTGGTCTAGTCAAAGCCTCGTA31120.2896947964782347No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23290.21680564942088967No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21900.2038661967504287No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21590.2009804195361532No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19950.18571372717676038No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTGGTCCCAGTTCCGAAGACGCC19670.1831072187251567No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19640.18282794996248491No Hit
GGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19350.18012835192332396No Hit
GTAATACACGGCCGTGTCGTCGGCTCTCAGGCTATTCATCTGCAGATACA19290.1795698143979803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19080.17761493305927759No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18920.17612549965836122No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18740.17444988708233028No Hit
AGGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17590.16374458451324386No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCC17460.16253441987499928No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT17140.15955555307316655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16140.15024659431743922No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16030.1492226088543092No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC15340.14279942731285736No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15140.14093763556171188No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15030.13991365009858187No Hit
GTCTGGGACCCCAGAGTCACGGTTAGAAACCTTAGAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA14850.13823803752255096No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTTCTGGACAGTCGATCACCATCTCCTG14470.13470063319537456No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14380.1338628269073591No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTT14320.13330428938201547No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTAATTTCACACTGAA13990.13023233299262546No Hit
GCCTTGGGCTGACCTTGGTCCCAGTTCCGAAGACGCCCTGCATATGAGCA13880.12920834752949542No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC13590.1265087494903345No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13510.12576403278987633No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13500.12567094320231903No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTCTAAGGTTT13330.1240884202138454No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCTGGTCTAGTCAAAGCCTCGTATA13260.12343679310094448No Hit
GCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA13100.1219473597000281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13060.12157500134979903No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12910.12017865753643991No Hit
CTTCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12790.11906158248575263No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12780.11896849289819537No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12680.11803759702262263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12610.11738596990972172No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12520.11654816362170627No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT12480.11617580527147717No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12370.11515181980834716No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12090.11254531135674349No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA12060.11226604259407169No Hit
AGTGTGTACCTTGCATGCAGGAATAAACCCCAACATCCTCAGCCTCCACC12060.11226604259407169No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12020.11189368424384259No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC11970.11142823630605622No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG11640.1083562799166662No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC11400.10612212981529164No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11370.10584286105261981No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11220.10444651723926072No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11220.10444651723926072No Hit
GTATACGAGGCTTTGACTAGACCAGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA11160.10388797971391708No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT11120.10351562136368798No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10930.1017469192000998No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC10850.10100220249964162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10780.10035057538674069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.047.0416451
GTATCAA19350.042.9655081
TGGTATC2500.041.3831562
GGTATCA7500.040.9366871
TATCAAC22150.037.366282
ATCAACG22250.037.3533363
TCAACGC22450.037.020574
CAACGCA23150.035.9028245
AACGCAG24050.034.559276
CCCCGTA500.003158632733.14664569
CGCAGAG25600.032.3290868
AGAGTAC25600.032.05967711
TCTGGAG9550.031.058570
CAGAGTA26700.030.86802310
GCAGAGT26900.030.7667149
GTATACC450.00462438930.6641831
GCGACGT450.004632822730.65276511
ATTAGTG13700.030.40250470
AGAGCAT5200.030.34049270
ACGCAGA27400.030.2052787
Produced by FastQC (version 0.11.5)