FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074234
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGGAATAAACCCCAACATCCTCAGCCTCCACCC37630.3502961179780197No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32570.30319278667403937No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCTGGTCTAGTCAAAGCCTCGTA24810.23095526672959524No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC22160.20628652602691777No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21590.2009804195361532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20050.1866446230523331No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18660.1737051703818721No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18280.17016776605469572No Hit
GGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18160.16905069100400844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17930.16690963049019117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17370.16169661358698384No Hit
GTAATACACGGCCGTGTCGTCGGCTCTCAGGCTATTCATCTGCAGATACA16850.15685595503400562No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCC16810.15648359668377654No Hit
AGGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16710.1555527008082038No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTGGTCCCAGTTCCGAAGACGCC16600.1545287153450738No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTTCTGGACAGTCGATCACCATCTCCTG16290.15164293813079832No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15800.14708154834049192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15740.1465230108151483No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC15480.14410268153865918No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15280.1422408897875137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15170.1412169043243837No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14970.1393551125732382No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14020.13051160175529725No Hit
GTCTGGGACCCCAGAGTCACGGTTAGAAACCTTAGAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA13870.12911525794193818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13680.12734655577834997No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCTGGTCTAGTCAAAGCCTCGTATA13570.12632257031521996No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13490.1255778536147618No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC13390.12464695773918905No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTAATTTCACACTGAA13340.12418150980140266No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13310.12390224103873085No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13170.12259898681292902No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12470.11608271568391988No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12460.11598962609636262No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12460.11598962609636262No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12330.11477946145811806No Hit
CTTCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12310.11459328228300351No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12110.11273149053185805No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA12070.11235913218162895No Hit
GCCTTGGGCTGACCTTGGTCCCAGTTCCGAAGACGCCCTGCATATGAGCA12060.11226604259407169No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12030.11198677383139985No Hit
AGTGTGTACCTTGCATGCAGGAATAAACCCCAACATCCTCAGCCTCCACC12000.11170750506872805No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11900.11077660919315531No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11890.11068351960559802No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11790.1097526237300253No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTT11180.10407415888903163No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10890.1013745608498707No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTCTAAGGTTT10870.10118838167475616No Hit
GCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA10840.10090911291208433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10800.10053675456185523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.045.4227181
TGGTATC2400.038.790222
ATTAGTG13600.037.61758470
GTATCAA17600.037.257661
TAAGACT853.0177034E-936.467659
GTGGTAT2650.036.4658931
TATCAAC20200.032.6025622
ATCAACG21050.031.2569333
ATTAGAG1001.4711986E-831.0032793
TCTGGAG9650.030.8697670
GTCGGGT450.00461235530.68051532
GTCGTAA450.00465143330.6276520
CAACGCA21900.030.0381745
AACGCAG21950.029.969756
TCAACGC21850.029.9478844
CGCAGAG22900.028.7264618
AGAGTAC23350.028.470511
GCGTTGT6550.027.789362
CAGAGTA23900.027.66862310
CGAACTA1005.017828E-727.5995929
Produced by FastQC (version 0.11.5)