FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578564
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC306.302811E-446.008933
GTATCAA10450.038.9712371
GGTATCA4900.035.2170031
CTCAACG501.751304E-434.85613353
ATACTCG400.002587314234.52161428
CGTATAC400.002592804634.50673
TATCAAC11950.033.78482
TCAACGC12500.032.2982674
ATCAACG12700.031.7896353
AACGCAG12700.031.7896356
CAACGCA12750.031.6649725
ACGCAGA13600.029.685917
AGAGTAC13800.029.00813711
CGCAGAG14650.027.558258
CAGAGTA14900.026.86427510
GCAGAGT15850.025.2541129
TAACGGC700.001314859824.7010136
CAAACGA1151.5751575E-624.20337349
GAGTACG13650.023.51208912
CGGCACA951.9397603E-423.3106868
Produced by FastQC (version 0.11.5)