FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578564
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA200.00737485851.718625
GTATCAA10400.041.8305851
TCAACGC11950.034.9118774
TATCAAC12250.034.658762
ATCAACG12150.034.355013
GGTATCA4350.034.1299741
CAACGCA12550.033.242785
AACGCAG12700.032.850156
ACGCAGA14000.029.7997787
AGAGTAC14550.028.91279611
CAGAGTA14700.028.61529410
GCCCGTT500.00691928628.23489666
TGCGAAA756.180149E-527.58565112
CGCAGAG15250.027.5832678
GCAGAGT16400.026.0695519
TATAAGA1752.382876E-1023.6693952
GTATAAG1902.5465852E-1123.6236211
TGGTATC1803.4015102E-1023.0119152
GAACCGT750.001998868822.9860556
TACTCCG1554.103822E-822.244575
Produced by FastQC (version 0.11.5)