FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538618
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.038.392551
GTATCAA47500.036.932141
CGTATGC1900.036.49176841
TCGTATG1900.034.63762340
ATCAACG55550.031.3906543
TCAACGC55600.031.3624254
TATCAAC56150.031.2997552
CAACGCA56900.030.6458855
AACGCAG58650.029.7899996
ACGCAGA61900.028.279527
CGCAGAG63400.027.4480328
TATGCCG2550.027.24807743
AGAGTAC63150.027.12187411
CAGAGTA64000.026.86892310
GTGGTAT7800.026.8480261
ACGAGAC2850.026.5014222
GCAGAGT66950.026.0951449
CTCGTAT2550.025.77883539
TCTCGTA2750.023.87741738
GAGTACG50400.023.83581512
Produced by FastQC (version 0.11.5)