FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538618
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20030.13018176051495564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48950.038.3107531
GGTATCA22000.033.565761
TATCAAC57900.032.428062
ATCAACG57500.032.2723733
TCAACGC57650.032.2374154
CAACGCA57950.032.070535
AACGCAG59300.031.6295436
GTGGTAT9350.031.2239631
ACGCAGA63100.029.7247547
CGCAGAG65750.028.578878
CAGAGTA64850.028.39479310
AGAGTAC65700.027.9257711
TGGTATC10500.027.787012
GCAGAGT68400.027.422419
GAGTACG54350.024.23050912
AGTACGG55000.023.8825713
GAGTACT24500.021.27690112
TACGGGG24300.020.74905815
AATAGCG3250.020.693862
GTACGGG63600.020.54598414
Produced by FastQC (version 0.11.5)