FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423340
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22780.16004608877710175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39650.039.1527021
GGTATCA15650.034.0160451
TATCAAC45650.033.378942
TCAACGC46250.032.9100344
ATCAACG46700.032.5320783
CAACGCA47100.032.2433325
AACGCAG48100.031.7155326
GTGGTAT8000.030.4813961
ACGCAGA51950.029.3651057
CGCAGAG52750.028.8547698
TACGATC500.006873247728.2763861
AGAGTAC53250.028.26293811
CAGAGTA54700.027.63713810
GCAGAGT56650.026.9278649
TGGTATC8700.026.0348452
GAGTACG41200.024.46887612
AGTACGG41850.024.09052713
GAGTACT23300.021.63342712
GTACGGG47950.020.88279714
AGTACTT24000.020.28950913
Produced by FastQC (version 0.11.5)