FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776180
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA25540.32904738591563815No Hit
GTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGGGCTGGATCCG18020.23216264268597492No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17680.2277822154654848No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16400.21129119534128682No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTGGGGGCTTTCCCTGGCT15670.20188616042670515No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCACTCTCACCATCAGCAGC15040.19376948645932643No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14660.18887371485995516No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAA14410.18565281249194773No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14310.18436445154474476No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGA14200.1829472545028215No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13620.1754747610090443No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12430.16014326573732898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12300.15846839650596511No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12050.1552474941379577No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA11940.15383029709603444No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11570.14906336159138345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11370.1464866396969775No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11090.14287922904480918No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT11080.1427503929500889No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGG10850.13978716277152206No Hit
GTAGTAGGTACTCCCACTATAATAGATACTCCCAATCCACTCCAACCCCTTCCCTGGGGGCTGGCGGATCCAGCC10680.13759694916127702No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10670.13746811306655674No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10440.1345048828879899No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10380.13373186631966813No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10050.12948027519389832No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9930.12793424205725476No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC9650.12432683140508646No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9650.12432683140508646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9550.12303847045788349No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9510.12252312607900229No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9490.1222654538895617No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9460.1218789456054008No Hit
CCTGTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC9330.12020407637403695No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9150.11788502666907161No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9130.117627354479631No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9130.117627354479631No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9120.11749851838491071No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGGCCAGTCAGA9080.11698317400602952No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8740.11260274678553943No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8720.11234507459609885No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8680.11182973021721765No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGGT8660.11157205802777706No Hit
GTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC8360.10770697518616816No Hit
GAATATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCCAAGGTCCTGATCTATAAGGCGT8340.10744930299672756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8230.1060321059548043No Hit
CTAATATTCTGACTGGCCCGGCAAGTGATGGTGAGTCTGTCTCCTACAGA8170.10525908938648253No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8130.10474374500760134No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8080.10409956453399985No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8000.10306887577623747No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7990.10294003968151719No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7840.10100749826071273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)