FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903687
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11830.1309081573597938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10380.11486277881611665No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9500.1051248939068505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.041.242981
GGTATCA9600.038.0843241
TATCAAC25950.037.0565952
ATCAACG26700.036.106763
TCAACGC26800.036.090514
CAACGCA27300.035.55565
AACGCAG28050.034.48226
ACGCAGA30000.032.1261187
AGAGTAC30300.031.9216411
CGCAGAG30400.031.703418
ACGACCG653.0636112E-431.6910269
AATAGCG1251.0186341E-1030.90774562
CAGAGTA31850.030.25840610
ATAGCGG1301.5825208E-1029.75594763
GCAGAGT32750.029.5319779
CGTTGAA1554.9112714E-1126.84297442
AGTACGG26500.026.76474213
GAGTACG26950.026.31492212
GTGGTAT5000.025.5236821
GAGTACT10150.025.43834312
Produced by FastQC (version 0.11.5)