FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310897
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46750.042.408931
GGTATCA18350.039.2718431
CAACGCA53650.037.0077135
TCAACGC53400.036.987324
ATCAACG54200.036.504983
TATCAAC57250.034.681342
AACGCAG57150.034.6206476
GTGGTAT7100.032.537751
ACGCAGA61150.032.4666377
CGCAGAG62800.031.5587278
AGAGTAC62750.030.75994911
CAGAGTA63450.030.47491810
GCAGAGT69750.028.3153349
TGGTATC8050.027.8334082
TCGCTAA1158.924246E-426.4010270
AGTACGG56250.025.0617913
GAGTACG56350.025.01731512
TATAAGA7050.023.9582922
AATAGCG5450.021.27070662
GTACGGG65550.021.24320214
Produced by FastQC (version 0.11.5)