FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310897
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50400.041.511081
GGTATCA21050.037.066221
TCAACGC57600.036.054014
CAACGCA57700.035.932615
ATCAACG58500.035.570523
TATCAAC60250.034.6872
AACGCAG60900.033.987246
ACGCAGA63250.032.778927
CGCAGAG65350.031.724218
AGAGTAC65650.031.1636311
CAGAGTA67300.030.34578310
GTGGTAT8250.030.1300931
GCAGAGT71650.028.9822189
TGGTATC8700.026.5586952
GAGTACG61550.026.07735612
AGTACGG61700.026.01677313
TACGGGG29750.024.31612615
CGCATAA2101.9525705E-623.61749670
GTACGGG70200.022.67031514
AGTACTT22450.019.64021913
Produced by FastQC (version 0.11.5)