FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786747
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63020.35270802189677664No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGGTCCTGAT45060.2521901533904912No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT41980.23495212248852243No Hit
GTCTCTGGTGGCTCCATCAGCAGTAATGATTACTACTGGAGTTGGATCCG37160.20797572347959728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36940.20674443555802807No Hit
GTAATACACGGCCGCGTCTGCGGCAGTCACAGAGGTCAGTTTCAGGGAGA35830.20053202831738348No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35250.1972859056150647No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG34440.1927525273583781No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33850.18945043702326073No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31910.17859271626033232No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT30540.17092515056692414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29780.16667161047423054No Hit
GTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29420.16465677569348094No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29070.16269790854552996No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28940.16197032931914815No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTTGGAGTGCTTTAGCCTGGTATCAGCAGAAA28810.16124275009276634No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT28700.16062710613198175No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28320.15850033608563496No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28120.15738098342966297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27340.15301550807137215No Hit
GTGTATTACTGTGCCAGCTCCTTCCCGGGCCTGCGACCCTTTGACAACTGGGGCCAGGGAACCCTGGTCACCGT27150.15195212304819877No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26080.1459635863387486No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT25680.14372488102680459No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24890.13930343803571518No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAATGATTACTACTGGA24840.1390235998717222No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24770.13863182644213198No Hit
GCTTAGTACTTTCCTTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT24070.13471409214622998No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG23660.1324194192014874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23600.1320836134046958No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC22690.1269905588200232No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21960.12290492162572542No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGACTTACCATATCAGTAGACACGTCCAAGAACCACTTCTCCCTGAAA21900.12256911582893382No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21810.12206540713374642No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21240.11887525206422621No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20930.11714025544746962No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG20780.11630074095549062No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19870.11120768637081803No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19670.11008833371484604No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18770.10505124676297203No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18770.10505124676297203No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18700.10465947333338184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5000.043.5118641
TACTGAT11750.041.2631370
GTATCAA31700.038.019231
CGTCGTA400.002528821434.68689344
TATCAAC36100.033.4547042
ATCAACG37200.032.2519233
TCAACGC37300.032.1573524
CAACGCA37600.031.8091135
AACGCAG38500.031.2445726
CGCAGAG41850.028.7435158
AGAGTAC42450.028.26316811
CTACTGA12700.028.13342969
CAGAGTA43300.027.70136810
GTCTACT11250.027.27593867
ACGCAGA44450.027.062237
GCAGAGT44600.027.0484929
CTGGTGT19350.026.72685470
TCTACTG12300.026.59958368
CGAGTAA809.575037E-525.87090321
GACGTGT12050.025.52850562
Produced by FastQC (version 0.11.5)