FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188432
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31600.039.8524481
GGTATCA14300.034.648741
TCGTATG905.011316E-934.62379540
TATCAAC38750.033.2065962
TCAACGC38850.032.9440044
ATCAACG38900.032.9016533
CAACGCA39700.032.240015
AACGCAG40300.031.760016
GTGGTAT5000.031.6554721
ACGCAGA44050.029.0501487
CGCAGAG45850.027.9096858
AGAGTAC46350.027.16216711
ACGCTAA657.6284114E-427.11952262
CAGAGTA47000.026.93289410
GCAGAGT48350.026.5366069
CGCTAAG851.2311051E-424.9302363
ACGTTAA700.001258058424.8915248
GAGTACG39500.024.81775712
AGTACGG39450.024.76202213
CTCGTAT1301.7956336E-723.955139
Produced by FastQC (version 0.11.5)