FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188432
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12200.1026562731397337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35300.038.0264741
ATCAACG41950.031.7135413
TCAACGC41950.031.540354
TATCAAC42850.031.306572
CAACGCA42450.031.168855
GTGGTAT7650.031.044441
AACGCAG43150.030.8225066
GGTATCA15850.030.6186451
ACGCAGA46200.028.7876857
TGGTATC8350.027.600062
CGCAGAG49000.027.212818
AGAGTAC48400.027.19856611
CAGAGTA48650.026.91523410
GCAGAGT51900.025.5598169
GTATTAA1501.0713848E-925.2404791
AGTACGG44250.023.46465713
GAGTACG44400.023.30598612
TACGGGT7100.021.79271315
CGCTTAT1004.4231815E-420.63825624
AGCGTAT850.00417683220.2463741
Produced by FastQC (version 0.11.5)