FastQCFastQC Report
Wed 8 May 2024
EGAF00005760526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309527
Total Bases98.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55300.4222898802392009No Hit
CTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA47960.36623910770835577No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40400.3085083392705916No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT38020.29033383809573987No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36170.2762065997875569No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34070.26017027522151126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT31970.24413395065546567No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGGTTCATGGAGA31780.24268304509949012No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC31400.239781233987539No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30980.2365739690743299No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29780.22741035503658957No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC28730.21939219275356672No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG27880.212901299476834No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGTACACTTTTGGCCAGG27280.2083194924579638No Hit
GTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGAGCTGGATCCG26080.19915587842022348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26060.19900315151959447No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25240.19274134859380523No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24640.18815954157493509No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCTGGCACTGATTTCACATTGAA23740.18128683104662982No Hit
GCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGAT23600.18021774274222677No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTG22710.17342139566423603No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT22610.172657761161091No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGT21800.16647232168561624No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21600.1649450526793262No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21580.1647923257786972No Hit
GTCTCCACTCTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCT21360.16311232987177815No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTG21300.16265414916989113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC20850.15921779390573848No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20070.15326144478120726No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGA19570.14944327226548212No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA19390.14806873015982105No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19170.146388734252902No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19110.14593055355101497No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18910.14440328454472492No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGA18890.14425055764409592No Hit
GTCTGGCACTGATTTCACATTGAAAATCAGCAGGGTGGAGGCTGAGGATG18850.14394510384283793No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18810.1436396500415799No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18760.14325783279000737No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAAGC18410.1405851120289998No Hit
GTGTATTACTGTGCGAGAGAAAGGCGTTCCTATGATATTAGTGGTTACCC18380.14035602167805628No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18210.1390578430227097No Hit
CTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCA17920.13684330296358915No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT17890.13661421261264564No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCATTGTGT17640.13470512635478307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17570.13417058220258155No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17430.13310149389817852No Hit
GTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCC17410.13294876699754948No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC17330.13233785939503348No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA17110.1306578634881144No Hit
GGTCTAGTCAAAGCCTCGTACACAATGATGGAAACACCTACTTGAATTGG16980.12966513863402587No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16580.12661060062144577No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16550.12638151027050226No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16460.12569423921767173No Hit
CTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA16420.1253887854164137No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16390.1251596950654702No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16200.12370878950949465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16150.12332697225792215No Hit
AGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGA15520.11851607488810845No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT15350.11721789623276191No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15250.11645426172961688No Hit
GTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTG14930.11401063131955277No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG14750.11263608921389173No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14710.11233063541263373No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14710.11233063541263373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14660.1119488181610612No Hit
CAATGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGT14660.1119488181610612No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14460.11042154915477116No Hit
GTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGA14110.10774882839376355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13670.10438883657992541No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13660.10431247312961092No Hit
ATCTATGACAGTGGGAGCACCTACTACAATCCGTCCCTCAAGAGTCGAGT13580.10370156552709489No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13520.10324338482520787No Hit
GTCATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGAGCTGGCGGA13400.10232702342143384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)