FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309527
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50380.3847190626844655No Hit
CTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA38200.29170838020140094No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC33440.2553593778516976No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC33440.2553593778516976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30370.23191579860514525No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28980.22130127901142932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28600.21839946789947823No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGGTTCATGGAGA27870.21282493602651947No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26710.20396677579003716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25940.19808679011582042No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25530.19495588865292582No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC24730.1888468126277656No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24470.18686136291958852No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.18021774274222677No Hit
GTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGAGCTGGATCCG23440.17899592753719473No Hit
GTCTCCACTCTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA22350.1706723114529139No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22150.16914504244662384No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21970.1677705003409628No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCTGGCACTGATTTCACATTGAA21470.16395232782523766No Hit
GCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21360.16311232987177815No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20320.15517053103906983No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA20240.1545596234365538No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT19990.15265053717869123No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19560.1493669088151676No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG19380.14799236670950655No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19010.14516691904786994No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18820.14371601349189442No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18260.13943966027428226No Hit
GTCTGGCACTGATTTCACATTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAG18090.1381414816189357No Hit
GTGTATTACTGTGCGAGAGAAAGGCGTTCCTATGATATTAGTGGTTACCC17840.13623239536107312No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17520.13378876495100903No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAATAGTGGTTACTACTGGA17520.13378876495100903No Hit
GTGCCAGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGGTT17250.13172695179251745No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC17190.13126877109063045No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17100.13058150003779992No Hit
CTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCA16800.1282905965283648No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16620.12691605442270376No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16430.12546514886672822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16360.1249306047145267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16220.12386151641012365No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16180.12355606260886565No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT16090.12286879155603513No Hit
GGCCAAAAGTGTACGGAGGCCAGTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTG15840.12095970529817256No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15820.12080697839754354No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15640.11943243629188248No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCATTGTGT15610.11920334594093898No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA15330.11706516933213289No Hit
AGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15240.11637789827930237No Hit
GTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGT15200.11607244447804438No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15180.11591971757741536No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15070.11507971962395583No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15030.11477426582269781No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14820.11317063336609326No Hit
GTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGC14810.11309426991577874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14470.11049791260508565No Hit
CTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA14340.10950518775099713No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14330.10942882430068261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14240.10874155324785209No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13880.10599246903653No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13690.10454156348055443No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13550.10347247517615138No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13460.10278520412332086No Hit
GGTCTAGTCAAAGCCTCGTACACAATGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGTCTC13440.10263247722269186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13280.1014106620176598No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13220.1009524813157728No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13150.10041793716357127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.043.8868871
GTATCAA25100.040.1431
GTGGTAT3350.036.0515481
TATCAAC28500.035.2073482
ATCAACG29500.033.9917873
TCAACGC29550.033.92524
TGGTATC3500.033.496242
AACGCAG30300.033.3128626
CAACGCA30200.033.3090975
TACTGAT11400.032.8602170
CGCAGAG33600.030.0410618
AGAGTAC34000.029.39040411
CAGAGTA34050.029.34276410
GCAGAGT34700.029.0876459
ACGCAGA35400.028.5135547
TTAACGG1653.6379788E-1227.23171635
TTTAACG1800.026.86921134
TCTATAC906.971126E-626.8014683
ACTACGC658.731638E-426.499868
TAACGGC1705.456968E-1226.44294436
Produced by FastQC (version 0.11.5)