FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081014
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22290.20619529441801865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15730.14551152899037384No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15060.13931364441163574No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT14540.13450334593261512No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13990.129415530233651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13960.1291380130137075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.12090500215538375No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12990.12016495623553441No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12900.11933240457570392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12380.1145221060966833No Hit
GAGTTACAGAACTCCTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11860.1097118076176627No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11480.10619658949837837No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11340.10490150913864205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT350.001379078439.24487311
GTATCAA26750.037.5434041
TATCAAC28800.034.13082
TCAACGC29600.033.408084
ATCAACG29750.033.247333
CAACGCA30700.032.2110525
AACGCAG31550.031.4520746
ATTAGAG1450.030.7911993
GACGCGT450.004731518730.52096610
GTGGTAT5650.029.827871
ACGCAGA34650.028.7372727
CGCAGAG34900.028.5300968
AGAGTAC35200.028.38842411
TATTAGA2150.025.577282
AATAGCG1401.0832082E-825.22351362
TAACGGC1251.2602322E-724.8484236
TTAACGG1251.2687633E-724.831135
CGGTTCG1401.3562385E-824.6959939
GGTATCA18850.024.631851
CGGTACC1001.4900666E-524.30054545
Produced by FastQC (version 0.11.5)