FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296596
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28790.22204294938438807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18420.14206429759153968No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18310.14121592230733399No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17880.13789954619634798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16440.12679354247583674No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16270.1254824170366097No Hit
GGGTATGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16190.12486541682991464No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15830.12208891589978682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15620.12046929035721228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15400.11877253978880083No Hit
ACCATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15000.11568753875532548No Hit
GTCTCTGGTGGCGCCATCAGAAGTGGTTATTACTGGAGTTGGATCCGCCA14710.11345091300605586No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14290.11021166192090674No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13400.1033475346214241No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAATCAGAGCATTAACAC13270.1023449092855446No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAATCAGAGCATTAACACCTATTTAAATTGGTATCAGCAGAAA13150.101419408975502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.042.6841351
TATCAAC30300.036.5713652
ATCAACG31450.035.014573
TCAACGC31950.034.5746574
CAACGCA32700.033.887235
AACGCAG33500.033.077986
TAGTCGT450.00394552231.67579367
AGAGTAC36800.030.29585611
ACGCAGA37000.029.9466727
TTAACGG1551.8189894E-1228.99640735
CAGAGTA39500.028.31238210
GTAGTTT4000.027.76595570
GTGGTAT4150.026.6193471
CGCAGAG42050.026.432318
CCGAAAT2250.026.22690647
AATAGCG2050.025.61865662
CGCGGTA1900.025.54462843
TAACGGC1851.8189894E-1124.30367336
CGAAATT2600.024.04169748
GAGTACG34750.023.9381612
Produced by FastQC (version 0.11.5)