FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296596
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26900.20746631950121705No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19260.14854279976183793No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16540.12756479273420557No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15650.12070066543472292No Hit
GGGTATGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15650.12070066543472292No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15030.11591891383283613No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14980.11553328870365172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14820.11429928829026158No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14370.1108286621276018No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14220.10967178674004856No Hit
ACCATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA14210.10959466171421167No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14180.10936328663670103No Hit
GTCTCTGGTGGCGCCATCAGAAGTGGTTATTACTGGAGTTGGATCCGCCA14120.10890053648167973No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13850.10681816078408388No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13840.10674103575824696No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAATCAGAGCATTAACACCTATTTAAATTGGTATCAGCAGAAA13780.10627828560322568No Hit
GTATGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13010.10033965861378563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29250.041.6592861
TATCAAC33200.036.7727052
GTGGTAT4050.035.7978741
ATCAACG34600.035.0543143
TCAACGC34700.034.8472754
CAACGCA35400.034.1582035
AACGCAG36350.033.2654886
AGAGTAC38600.030.88495311
ACGCAGA39700.030.4584487
CAGAGTA41650.028.70267710
CGCAGAG43850.027.418718
GGTATCA20300.025.3368841
AGTACGG34300.024.61769713
GAGTACG34800.024.35833212
GAGTACT14350.023.77250512
GTATTAG2200.023.5359421
GCAGAGT50900.023.4856649
AGTACTT14450.023.37403513
GTATTAC1202.4462715E-623.012921
TTCGGGG8200.022.93916770
Produced by FastQC (version 0.11.5)