FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518837
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14120.27214712905980104No Hit
CCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG10080.19428067003702512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9650.18599290335885837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9300.17924704676035055No Hit
GTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTGCCAGATCCACTGCCG8990.1732721452016722No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8690.16748998240295124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8430.1624787746440597No Hit
ACCTATTACTGTCTACAAGATTACAGTCACCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8060.15534744052563715No Hit
GTCCATGGGTGACTGTAATCTTGTAGACAGTAATAGGTTGCAAAATCTTC7960.15342005292606348No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7950.1532273141661061No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7740.14917980020700142No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7610.14667419632755566No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7480.14416859244810992No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7230.13935012344917574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6850.13202605057079583No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6480.12489471645237328No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCTACAAGATTACAGTCAC6370.12277459009284228No Hit
ATATTGCATCCACTTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGAATTCACTCTCA6360.12258185133288489No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6160.11872707613373758No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6010.11583599473437708No Hit
AGATTACAGTCACCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5960.11487230093459025No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTGACCTGGTATCAACAGAAA5870.11313765209497394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5570.10735548929625296No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5510.10619905673650877No Hit
GATCTATATTGCATCCACTTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGAATTCA5490.10581357921659404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5330.10272975905727619No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT5290.10195880401744671No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5220.10060963269774516No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5190.10003141641787305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG200.006903961852.5879858
TCGTATG200.007253451351.93540640
GTGGTAT1400.041.7998121
CTAGACT451.0145715E-438.244514
TCAACGC15950.033.6647574
ATCAACG16050.033.4550063
CAACGCA16300.033.153065
AACGCAG16650.032.456156
GTATCAA17900.031.1541161
GTACTAG450.00467090530.5985551
GTATTCC450.00467310830.5956087
ACTAGAC450.00467310830.5956083
AGAGTAC18200.029.88114711
ACGCAGA18200.029.6920267
GGTATCA9750.029.6570591
CAGAGTA18500.029.58264210
CGAATTA604.66044E-429.4993365
CGCAGAG18650.029.3447138
AGTACGG14200.029.32976713
TATCAAC19200.028.8626522
Produced by FastQC (version 0.11.5)