FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518837
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12020.23167198946875417No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9410.1813671731198816No Hit
GTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTGCCAGATCCACTGCCG8860.17076654132222646No Hit
ACCTATTACTGTCTACAAGATTACAGTCACCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8810.16980284752243963No Hit
CCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG8800.16961010876248225No Hit
GTCCATGGGTGACTGTAATCTTGTAGACAGTAATAGGTTGCAAAATCTTC8300.15997317076461393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8260.15920221572478446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7990.1539982692059356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7910.15245635912627664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7890.15207088160636192No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7360.1418557273286215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7330.14127751104874942No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7220.1391573846892184No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7170.13819369088943156No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCTACAAGATTACAGTCAC6860.1322187893307532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6570.1266293652919896No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6480.12489471645237328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6450.12431650017250119No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6310.12161815753309806No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTGACCTGGTATCAACAGAAA6040.11641421101424919No Hit
ATATTGCATCCACTTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGAATTCACTCTCA5780.11140300325535767No Hit
AGATTACAGTCACCCATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5670.10928287689582664No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5510.10619905673650877No Hit
GATCTATATTGCATCCACTTTACAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGAATTCA5480.10562084045663667No Hit
GTTTAGGGGCTGTCCCTGGTTTCTGTTGATACCAGGTCAAATCATTTCTA5200.10022415517783041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.039.597091
ATCAACG14750.035.4245723
GTCATAT400.002510337234.73414645
TCAACGC14850.034.7096674
GTACTAG400.002620155734.4322551
GCCCGAT400.002643818534.36919412
CAACGCA15150.034.0223435
AACGCAG15250.033.7992486
ACGCAGA15950.032.3158957
CGCAGAG16450.031.124768
AGAGTAC16500.031.0334311
CAGAGTA16600.030.84350810
GCAGAGT17800.028.7641749
CGAACCG854.436406E-628.39985833
GTATCAA18450.027.9937041
TCGCTAT500.00784611327.51657324
CTCGCTA500.00784611327.51657323
AATAGCG1056.401733E-726.83533762
AGTACGG15250.026.37103713
CCTAGTC809.737718E-525.8017652
Produced by FastQC (version 0.11.5)