FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015029
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33570.3307294668428193No Hit
GTGTAATCAGTGGCCTCCGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26290.2590073781143199No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG24090.2373331205315316No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGGAGCCTGCAGTCTGAAGATT22230.21900852093881062No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21870.2154618242434453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21020.20708767926827706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19850.19556091500833966No Hit
GAGTTCACTCTCACCATCAGGAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAATCAGTGG18610.18334451527985898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18010.17743335412091674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17220.16965032526164278No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16970.16718734144541683No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15920.1568428094172679No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15640.15408426754309484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15620.15388722883779676No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC15330.15103016761097465No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14690.14472492904143625No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14280.1406856355828257No Hit
CTGATTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTCCTGATGGTGAGAGTGAACT14050.13841969047189784No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13890.13684338082951325No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13760.13556262924507576No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAG13640.1343803970132873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13050.1285677552069941No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12910.12718848426990756No Hit
GCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC12780.1259077326854701No Hit
GTGTTAGGAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGCGCATCCA12190.12009509087917686No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12090.11910989735268648No Hit
CATCTATGGCGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA11650.11477504583612883No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10660.10502162992387408No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10620.10462755251327795No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10390.10236160740235009No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGACCTGAGCTTCAGGGAGAACTGGTTCTCAGACGTCTCTACTGA10390.10236160740235009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25600.041.345981
GGTATCA10800.041.1813771
TATCAAC28500.037.127872
ATCAACG29550.035.575323
TCAACGC29700.035.3973924
CAACGCA30200.034.8113445
AACGCAG30600.034.3562976
GTGGTAT3950.033.1681631
AGAGTAC32800.031.83859611
CGCAGAG33500.031.2777358
GCAGAGT34700.030.295419
CAGAGTA35400.029.6963510
AGTACGG29350.027.94820813
ACGCAGA37700.027.8846387
TACGATT500.007683028527.63778337
GAGTACG29750.027.57243512
TACGGGG16900.023.65682215
TGTGCGA2650.023.4117110
TCGAGTA901.9026153E-423.38013562
GTACGGG34850.023.3396514
Produced by FastQC (version 0.11.5)