FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161695
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT2400.1484275951637342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAA150.002403265368.6248861
TATACAC353.4094774E-758.7849433
TTACTGC200.007120252.16287644
AAACGCT200.007526128651.43682517
CCGGACA250.00914069748.96437569
CGTATGC451.7832135E-646.2509441
CTCGTAT451.8025312E-646.17869639
ATAAGAG306.4864336E-445.72162217
TCGTATG503.697598E-641.5998140
TCTCGTA503.7536447E-641.50895738
CTTATAC503.9682036E-641.174931
GCCGTCT655.035563E-737.4973246
ACGAGAC557.65159E-637.42017422
ATCTCCG557.667861E-637.408610
GTATCAA5850.035.7787861
TATGCCG652.1963931E-532.08010543
AGGGGGG750.003699049432.0646470
TATCAAC6650.031.9806482
TTATACA652.4020314E-531.6632212
ATACACA652.4071202E-531.6534294
Produced by FastQC (version 0.11.5)