FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161695
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5400.3339620891184019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAT350.006425535353.51499670
ACTCCGT200.007011723752.3657251
TGATACT200.007476656751.5226827
GTTTATC200.007504161451.474878
GTATCAA4500.041.2564621
TCAACGC5400.033.6810874
TATCAAC5350.033.4164472
ATCAACG5500.033.099423
AACGCAG5700.031.3063536
GTACCTC553.3477804E-431.1968911
CAACGCA5750.031.0341245
CAGAGTA5750.030.43731510
ACGCAGA5850.029.9170177
AGAGTAC5950.029.4142111
TTTCGTC605.143264E-428.99171645
GGTATCA1800.028.6503221
CGCAGAG6150.028.4576518
GTGGTAT1103.7465725E-828.1294041
TACCATC500.00791891927.4532647
TACTATG500.00791891927.45326417
Produced by FastQC (version 0.11.5)