FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126051
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75080.35314298669222893No Hit
GTATAATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC74690.3513085998407376No Hit
GTATTATACTTATTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT73590.34613468820832616No Hit
GTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGA71870.3380445718376464No Hit
GTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA62590.2943955718842116No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT56550.2659860934662433No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54430.2560145546837776No Hit
CACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGG51300.2412924243115523No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC50970.23974025082182882No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC49950.23494262367177457No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG49670.2336256279835244No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49010.23052128100407748No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT48750.22929835643641663No Hit
CTATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGA47870.22515922713048747No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47380.22285448467604962No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA47180.22191377347015664No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46970.220926026703969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43840.2062038963317437No Hit
CAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGA43600.20507504288467207No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43490.20455765172143095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42830.20145330474198408No Hit
ATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCA42770.2011710913802162No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG42690.200794806897859No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41450.19496239742132243No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40730.19157583708010767No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAC40520.19058809031392004No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG39770.18706042329182132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39460.18560232092268716No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38650.18179244053882057No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37690.1772770267505342No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36940.1737493597284355No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA36930.17370232416814085No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT36370.17106833279164047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35490.1669292034857113No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA35360.16631774120188086No Hit
GTCTCGCACAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGG34200.1608616162077015No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC33870.15930944271797806No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAG33850.15921537159738877No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC33500.15756912698707604No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT33100.15568770457529005No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32860.15455885112821846No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC32510.15291260651790572No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32440.15258335759584318No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT31630.1487734772119766No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30730.1445402767854581No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT29650.13946043627363597No Hit
GTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCACTGTCTCCT28550.1342865246412245No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA28460.13386320459857265No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA28110.13221695998825994No Hit
GTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAGCTCTGTGACCGCCGCAGACACGGCTCTATATTA27740.1304766442573579No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27490.12930075524999166No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27250.12817190180292007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27000.1269960127955538No Hit
GTAGTAGGTGGTCCCACCGTAATAGATACTGCCAATCCAGTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC26960.12680787055437523No Hit
ATCTATTACGGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGT26870.12638455051172337No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT26820.12614937271025012No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26560.12492644814258924No Hit
CCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTAT26130.12290391904991932No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25750.12111656775872263No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC25450.11970550094988315No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25100.1180592563395704No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC24860.11693040289249881No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24560.11551933608365933No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC24380.11467269599835564No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG24150.1135908781115787No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24070.1132145936292215No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23920.11250906022480175No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23850.11217981130273921No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA23830.11208574018214991No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT23440.11025135333065858No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG23320.10968692660712279No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22990.10813475311739935No Hit
GCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG22900.10771143307474751No Hit
GAGTGAACTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA22810.10728811303209566No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG22700.10677072186885452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22550.10606518846443477No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22520.10592408178355082No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22500.10583001066296152No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACT22420.1054537261806043No Hit
TTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTATCTATTACGGTGGGAC22410.10540669062030968No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22300.10488929945706851No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCC22170.10427783717323809No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG21900.10300787704528255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21890.10296084148498791No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC21810.10258455700263071No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21610.1016438457967377No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC21500.10112645463349655No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21430.10079720571143401No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC21410.1007031345908447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.045.5217321
GTATCAA32300.037.179341
TATCAAC36900.032.633432
CCGGTTG800.003679418532.13055470
ATCAACG39050.030.9250683
TCAACGC39150.030.7579464
CAACGCA39850.030.3056645
AACGCAG40650.029.879016
GTAGTTT2252.8812792E-928.5604970
TTCGGGG31700.026.96128370
AGAGTAC44450.026.93337211
CGCAGAG45400.026.5983948
GCAGAGT46200.026.13729
ATCAGGC22400.026.08014936
TGTTAGA12100.025.49200870
CTACGGA23050.025.37050869
AATCATC24150.024.31222232
ACGCAGA50700.024.0900947
AGCGCTG10050.024.0334455
AAATCAT24550.023.91159431
Produced by FastQC (version 0.11.5)