FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126051
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC76170.35826986276434575No Hit
GTATTATACTTATTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT73250.34453547915830807No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73140.3440180879950669No Hit
GTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGA71550.3365394339082176No Hit
GTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA62790.2953362830901046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT59100.27798016134137893No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT51880.2440204868086419No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51500.24223313551744527No Hit
CACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGG49420.23244973897615814No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA48000.2257706894143179No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC46630.2193268176539509No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG45580.21438808382301272No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT45560.21429401270242343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43980.2068623941758688No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43770.20587464740968114No Hit
CTATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGA42890.20173551810375198No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42000.19754935323752815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41800.19660864203163517No Hit
CAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGA41700.19613828642868866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41510.19524461078309033No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41240.1939746506551348No Hit
ATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCA40660.19124658815804513No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG39370.18517900088003533No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39240.1845675385962049No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39010.18348572070942795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39000.1834386851491333No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAC37940.17845291575790045No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37150.17473710649462312No Hit
GAGTGAACTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA36810.17313789744460506No Hit
GTCTCGCACAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGG34830.16382485650626444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34650.16297821642096075No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA34510.16231971857683564No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG33620.15813355371061183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33380.15700470026354024No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT33000.15521734897234357No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC32950.15498217117087032No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT32950.15498217117087032No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA32740.15399442440468267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32360.152207073113486No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32180.1513604330281823No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30980.14571616579282437No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT30770.14472841902663672No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC30400.14298810329573466No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT30390.14294106773544002No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT30220.14214146321043097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29790.14011893411776105No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29630.13936636515304665No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAG29320.13790826278391252No Hit
GTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCACTGTCTCCT28210.13268731559120642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28020.13179363994560808No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA27960.13151142658384019No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27930.13137031990295625No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT27920.13132328434266158No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT27720.13038257313676857No Hit
GTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAGCTCTGTGACCGCCGCAGACACGGCTCTATATTA26760.12586715934848222No Hit
GTAGTAGGTGGTCCCACCGTAATAGATACTGCCAATCCAGTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC26490.1245971992205267No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT26470.1245031280999374No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA25820.12144581668078516No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25690.12083435439695474No Hit
ATCTATTACGGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGT25140.118247398580749No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24990.11754186517632925No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA24880.11702447401308813No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA24830.11678929621161487No Hit
CCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTAT24790.11660115397043626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24390.11471973155865028No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24350.11453158931747169No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23970.112744238026275No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23680.11138020677773017No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG23660.11128613565714086No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23430.11020431777036394No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23330.10973396216741743No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23200.10912249988358699No Hit
GCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG22940.1078995753159261No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22890.10766439751445285No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC22860.1075232908335689No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC22830.10738218415268495No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCC22790.10719404191150636No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC22680.10667665074826521No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG22190.10437190829382739No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT21990.1034311970879344No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACT21920.10310194816587186No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA21830.10267862812321998No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21500.10112645463349655No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC21490.1010794190732019No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA21440.10084424127172865No Hit
TTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTATCTATTACGGTGGGAC21270.10004463674671964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5300.046.2183071
GTATCAA31200.039.2559361
GCCCGAT951.8792323E-434.44402370
TATCAAC36850.033.0303422
CGCATCG800.003365041532.72182570
ATCAACG38400.031.7666853
TCAACGC39100.031.0995984
CAACGCA39950.030.8690385
AACGCAG40800.030.057076
AGAGTAC44250.027.7142911
CGCAGAG44600.027.6506278
GCAGAGT45800.026.9261579
TCCTATA906.9398593E-626.8183082
GCTTGTA2400.025.8757421
AGGTACG700.001338580224.6290592
ATCAGGC24450.024.59050636
ACGCAGA50350.024.3560777
CTACGGA22700.024.3280269
CAGCGCT9700.023.7935854
AGCGCTG9700.023.7935855
Produced by FastQC (version 0.11.5)