FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470442
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23180.15763967568935056No Hit
GTCTACGGTGACTGCGGTCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAACCTCCACCAAGGG19550.13295322086828315No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGG18590.12642457165940582No Hit
GTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG17150.11663159784608983No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16190.11010294863721248No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15250.10371031295352011No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGA14870.10112605597500615No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14850.10099004244982122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.039.7007181
TATCAAC28100.034.533292
GTGGTAT4000.033.6802641
TCAACGC30250.032.1930164
CAACGCA31050.031.5860025
AACGCAG31250.031.2733486
AGAGTAC33950.028.68450211
ACGCAGA34750.028.222897
CGCAGAG35200.027.7639798
CAGAGTA35250.027.62663310
TTAACGG1404.5474735E-1027.17673335
GCAGAGT35900.027.1264279
ATCAACG36850.026.333393
GAGTACG34550.023.68853812
AGTACGG34550.023.58858513
ATTAGAG1803.4015102E-1023.0221943
TACGGTG7200.022.5425644
TTTAACG1704.321919E-922.37626634
GCGTAGA13300.021.55800670
TCTACGG8200.021.478022
Produced by FastQC (version 0.11.5)