FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470442
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21340.14512643137233566No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGG19090.1298249097890294No Hit
GTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG18430.12533646345792626No Hit
GTCTACGGTGACTGCGGTCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAACCTCCACCAAGGG18060.12282021324200479No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17070.11608754374535003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.039.6764451
GTATCAA28400.038.1810761
TATCAAC31750.034.2402882
TCAACGC32800.032.7849854
CAACGCA33050.032.6412735
AACGCAG33850.031.869846
GTACTAG901.9790605E-730.696181
AGAGTAC37000.028.88106711
ACGCAGA37700.028.798077
CGCAGAG38500.028.1996738
CAGAGTA38200.028.06023410
AACGCGA500.007764889427.57863812
ATCAACG39650.027.2153263
GCAGAGT39650.027.2079229
AGTACGG34650.023.58154513
GAGTACG35400.023.07958212
GAGTACT12150.020.7123512
AATAGCG2201.5643309E-1020.69151762
GGTATCA23050.020.6749761
TTCGGGG10000.020.4698870
Produced by FastQC (version 0.11.5)