FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988643
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15270.15445413561821608No Hit
GTACTGAGACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12330.12471640420252811No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11480.11611876076601968No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11380.11510727330290105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.041.352931
GTATCAA27650.037.6388971
TATCAAC30400.034.098242
TCAACGC31400.032.6469084
ATCAACG31550.032.394133
CAACGCA32550.031.5991675
AACGCAG33350.031.047466
TAGCGAT2800.030.60095870
ACGCAGA35950.028.800577
CGCAGAG36250.028.562228
AGAGTAC36800.028.23166711
GCAGAGT38800.026.5964099
CTAGCGA2650.026.49794869
CAGAGTA39850.026.15722710
AGTACGG31850.025.81854613
GAGTACG32600.025.2245612
GGTATCA19150.025.0133081
AGGTACG700.001347744824.5986352
CGTCACA3203.5106495E-1022.95071870
ACGGGAT3000.022.9424616
Produced by FastQC (version 0.11.5)