FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144532
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2890.19995571914870064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATA100.008658994135.5393770
ACGTCAG150.002269206669.6207843
GCTGACA300.002520138967.76968470
CCATTGA200.005986396754.4967666
ACTTTGC200.00651154953.35112456
AACAATG200.00737283751.70205737
ATAGGTC200.00782322650.9327859
TAACCCT200.00782322650.9327854
ACTGTTT200.00782322650.93278511
CTGACCC306.783308E-445.3049421
ATGAAAT350.001156736440.64847656
GCTCTGA350.001440957938.87318418
GGTATCA3950.038.7636071
GTATCAA9150.037.1867031
TGGTATC1250.035.3378522
TATCAAC9750.034.5014572
GGATAAC400.0027706134.0258331
GTGTATA400.0027706134.0258331
GGTATCT400.002775326134.01403819
TGTATAA400.002789512533.9787062
Produced by FastQC (version 0.11.5)