FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708649
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35400.043.8378071
TATCAAC41800.036.9290242
TCAACGC41850.036.7657624
ATCAACG42300.036.388483
CAACGCA42500.036.2835735
AACGCAG44100.035.045396
GGTATCA14600.035.0360071
ACGCAGA47850.032.370987
CGCAGAG48950.031.4321178
AGAGTAC48350.031.25228911
CAGAGTA49350.030.89863410
GCAGAGT53350.028.904449
AGTACGG41300.026.4828213
GAGTACG42150.026.1943412
GTGGTAT6200.024.5282571
GTACGGG48500.022.05401414
AGTACTT18800.021.65604613
GAGTACT19350.021.2188112
GTATAGG3600.021.1215551
TGGTATC7050.021.062792
Produced by FastQC (version 0.11.5)