FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275997
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTTGAGCCTGAAGATT26140.20485941581367353No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23210.18189697938161298No Hit
GTCCCAGACCGACTGCCACTGAACCTGGCAGGGATGCCAGTGGCCCTGTT18820.14749250977862802No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18640.1460818481548154No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14950.11716328486665721No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14400.11285292990500763No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC13350.10462407043276747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13060.10235133781662496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12810.1003920855613297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.042.1346631
GTATCAA35700.035.967281
GTGGTAT7600.032.1596871
ATCAACG41300.031.0489233
TATCAAC41650.030.644552
TCAACGC41850.030.6312664
CAACGCA42400.030.2327425
AACGCAG43100.029.8214596
TGGTATC8150.029.1263352
AGAGTAC45700.027.98101211
ACGCAGA46500.027.6409687
CGCAGAG47300.027.100818
CAGAGTA48550.026.47487610
GCAGAGT48950.026.117099
ATACGGC700.001361614424.556123
GAGTACG41450.024.2994712
AGTACGG41750.024.12769913
TACGGGG23700.021.9070815
GTACGGG48000.020.91444814
ACCGCGT850.00421400120.2155558
Produced by FastQC (version 0.11.5)