FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740492
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20030.115082401987484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57650.039.3411561
GGTATCA24350.034.011091
TATCAAC67900.033.2284932
TCAACGC69750.032.258034
ATCAACG70350.031.9470023
CAACGCA71300.031.5567675
AACGCAG72950.030.8900246
GTGGTAT10850.030.4049281
ACGCAGA78650.028.5641167
CGCAGAG80050.028.0209038
AGAGTAC79150.027.95274211
CAGAGTA79600.027.8354110
GCAGAGT83750.026.7829639
TGGTATC11400.025.9064922
AGTACGG60200.025.7051713
GAGTACG60800.025.44638312
TAGGACC4150.022.3148424
GTACGGG71850.021.25072114
GAGTACT34250.019.330812
TACGGGG32400.019.27543615
Produced by FastQC (version 0.11.5)