FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702345
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA26870.3825755148822872No Hit
GTATAATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC25240.35936754728801373No Hit
GTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGA24640.35082473713061246No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22350.3182196783631976No Hit
GTATTATACTTATTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT21100.30042215720194493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18710.2663932967416298No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18240.2597014287849988No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18010.25642668489132836No Hit
CAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGA17830.253863841844108No Hit
CACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGG17280.24603293253315678No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17070.24304294897806636No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16630.2367782215293054No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16450.234215378482085No Hit
CTATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGA16260.2315101552655746No Hit
ATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCA15700.2235368657853334No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14130.2011831792068001No Hit
GTCTCGCACAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGG13920.19819319565170962No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13600.19363703023442896No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAC12900.18367041838412745No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12700.18082281499832703No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12440.17712093059678646No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12030.1712833436558956No Hit
GAGTGAACTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA11760.16743907908506503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11740.16715431874648498No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT11740.16715431874648498No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11610.1653033765457147No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA11490.16359481451423447No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11410.16245577315991427No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11230.1598929301126939No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11210.15960816977411385No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11130.1584691284197937No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10850.15448248367967307No Hit
GTAGTAGGTGGTCCCACCGTAATAGATACTGCCAATCCAGTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC10650.15163488029387268No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10620.1512077397860026No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10510.14964155792381237No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10490.14935679758523232No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10480.14921441741594232No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10440.14864489673878223No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.14750585538446206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10260.14608205369156183No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10250.14593967352227183No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10200.1452277726758217No Hit
GTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCACTGTCTCCT10120.14408873132150155No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10110.14394635115221152No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10100.1438039709829215No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9690.13796638404203063No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9660.13753924353416055No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9580.1364002021798404No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9520.13554592116410027No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9440.13440687980978008No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9340.1329830781168799No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9320.13269831777829982No Hit
CCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTAT9310.1325559376090098No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACT9190.13084737557752954No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9150.13027785490036947No Hit
ATCTATTACGGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGT9080.12928119371533933No Hit
GTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGAGCTCTGTGACCGCCGCAGACACGGCTCTATATTA9080.12928119371533933No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT8960.12757263168385907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8840.1258640696523788No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8720.12415550762089857No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA8620.12273170592799834No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8610.12258932575870832No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACGTCC8480.12073838355793805No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC8470.12059600338864802No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8450.12031124305006799No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTA8390.11945696203432786No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8260.11760601983355759No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8190.11660935864852744No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8180.11646697847923743No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG8170.11632459830994739No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG8100.11532793712491723No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8060.11475841644775717No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC7870.11205319323124675No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTT7810.11119891221550662No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7810.11119891221550662No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7790.11091415187692658No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC7790.11091415187692658No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC7700.10963273035331639No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA7690.10949035018402638No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCA7690.10949035018402638No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA7660.10906320967615629No Hit
TTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGACTGGATTGGCAGTATCTATTACGGTGGGAC7520.107069887306096No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCCTCGGCCCCAGAGATCGAAGTA7490.10664274679822595No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAG7460.10621560629035587No Hit
GTCTACGGACATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGGTCCCACCGTAATAGATACTGCCAAT7380.1050765649360357No Hit
GGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGAT7320.10422228392029557No Hit
GGGCTGGACTGGATTGGCAGTATCTATTACGGTGGGACCACCTACTACAA7310.10407990375100556No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT7140.1016594408730752No Hit
GGGTGCCTCGGCCCCAGAGATCGAAGTACCCGTGTCGGTAGTCACCGCCC7140.1016594408730752No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA7090.10094754002662508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT309.103467E-657.5200772
GTGGTAT1650.052.3208161
TCGGATT200.00729193751.86789337
GTATCAA11150.039.951521
GTGCTAA350.00134138239.4648441
TCCTAAT350.00134514939.4423372
GCGGGAC400.002563247234.58847438
CGCTGAT400.002599371634.48991816
TATCAAC13400.033.224282
ACTATAC652.2911236E-531.90503335
TTACCCC652.3316847E-531.8232484
CTATACT553.2152742E-431.43059736
GTCCTAA553.238234E-431.392491
ATCAACG14200.031.330163
CGAACTA450.004607157330.68612329
AGCGAAC450.00461519930.6751927
GCGTCCC450.004632928430.65115712
ACTCGGT450.00463615830.64679111
CAACGCA14550.030.5656285
TCAACGC14550.030.5656284
Produced by FastQC (version 0.11.5)