FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275564
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96350.036.6648331
TATCAAC111150.032.5090872
GGTATCA43850.031.9925271
ATCAACG116050.031.0779253
TCAACGC116900.030.76484
AACGCAG118150.030.4393146
CAACGCA119000.030.2789675
ACGCAGA127850.028.0989937
AGAGTAC130550.027.33579311
CGCAGAG131450.027.277798
CAGAGTA131150.027.15895510
GCAGAGT136700.026.205349
ACGGGTA5500.025.92996816
AGTACGG93600.025.50265113
GAGTACG95350.025.14142212
GTGGTAT18700.024.885541
GTACGGG102700.023.11067214
TACGGGG36050.022.7000715
ACGCGTA1804.110916E-1022.67114827
CGCGTAG2157.2759576E-1222.16049428
Produced by FastQC (version 0.11.5)