FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275564
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27150.11931108068153655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100150.034.835321
TATCAAC113600.030.6940062
GGTATCA48050.029.551751
ATCAACG119550.029.0846143
TCAACGC120000.028.998084
AACGCAG121650.028.6605266
CAACGCA122100.028.4993425
GTGGTAT21900.028.3862041
ACGCAGA128800.027.069517
AGAGTAC129650.026.76418311
CGCAGAG130350.026.6695658
CAGAGTA131700.026.42194210
GCAGAGT134700.025.8586589
AGTACGG99300.025.21578613
GAGTACG101050.024.91175512
TGGTATC25850.024.0354352
ACGGGTA5350.023.46615616
GTACGGG111800.022.06266814
TACGGGG39200.021.72514215
GTATATA2451.8189894E-1220.798191
Produced by FastQC (version 0.11.5)