FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213080
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC51800.4270122333234412No Hit
GTATTATACTTATTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCGAACGAACTGTGGCTGCACCAT49710.40978336136116333No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38790.31976456622811356No Hit
GTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA26740.22043063936426285No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT25780.21251689913278599No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23130.1906716787021466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23120.19058924390806872No Hit
GTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGA22980.18943515679097833No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA22120.18234576450028028No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21960.18102680779503413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20760.171134632505688No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20330.16758993636033898No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19670.1621492399511986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19540.1610775876281861No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19390.15984106571701784No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18850.1553895868368121No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18680.15398819533748803No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18160.14970158604543807No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18160.14970158604543807No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18070.1489596728987371No Hit
CTATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGA18040.14871236851650343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17440.14376628087183038No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17210.14187028060803902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16850.1389026280212352No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16370.13494575790549676No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAG16260.13403897517064003No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16250.13395654037656216No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15880.1309064529956804No Hit
GAGTGAACTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15710.12950506149635638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15670.12917532232004483No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15580.1284334091733439No Hit
CAGTAATATAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGTTCAGGGA15570.12835097437926601No Hit
CACCTGCACTGTCTCTGGTGGCTCCCTCAGCAGTGATCATTACTACTGGG15240.1256306261746958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15160.12497114782207275No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15110.12455897385168332No Hit
ATATTACTGTGCGAGACAGGGCGGTGACTACCGACACGGGTACTTCGATCTCTGGGGCCGAGGCACCCTGGTCA14840.12233323441158044No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14270.11763445114914103No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14090.11615062485573911No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14060.11590332047350545No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13630.11235862432815644No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13400.11046262406436509No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13360.11013288488805355No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT13340.10996801529989779No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13100.10798958024202857No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13020.10733010188940548No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12960.10683549312493817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12840.10584627559600357No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12690.1046097536848353No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12590.10378540574405647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCTTGGTCC12540.10337323177366702No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT12350.10180697068618723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12330.10164210109803146No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12180.10040557918686319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.046.98641
GTATCAA30650.040.0914731
TATCAAC34500.035.3021662
ATCAACG36650.033.209313
TCAACGC37400.032.7204634
CAACGCA37750.032.5081525
AACGCAG38550.031.8335346
TAGGCGC450.004705690330.5554394
GTAGTTT2351.4188117E-1030.04079270
ACTTTCG14900.030.01747923
AGAGTAC41600.029.6648511
CGCAGAG41750.029.4759358
TTTCGGC15400.028.83137525
GCAGAGT43400.028.5125399
CTACGGA9100.028.50760769
TTCGGCG15500.028.43035126
CTTTCGG15800.028.31463224
TTCGGGG10200.028.31384870
CGGAGGG15950.028.30967131
AGCGCTG4150.028.162545
Produced by FastQC (version 0.11.5)