FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872586
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA30600.35068176661097017No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27400.31400916356668573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26060.29865251104189156No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22490.25773963827061175No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20940.23997634617103644No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG20860.23905953109492933No Hit
GTATATTACTGTGCGAGAGAGTTTGGGCATTACGATATTTTGACTGGTCCCCTTGACTCCTGGGGCCAGGGAACC20070.23000598221837162No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19960.22874536148872432No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18290.20960684677498836No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18140.20788781850728752No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17460.20009489036037711No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16900.1936771848276273No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG16790.19241656409798003No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16610.190353730176739No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16290.18668646987231058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15940.18267540391434198No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15890.182102394491775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.17992495868602065No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAAAAACCAGGGAA15540.1780913285338064No Hit
GTATGATAATCTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15510.17774752288026624No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCATCATCTCCAGAGACAACGCCCAGAATTTGCTGTATCTGCAAATGAA15390.1763723002661056No Hit
CTCCTGTGCAGCCTCTGCATTCAGCTTTAGTTCCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15390.1763723002661056No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14770.16926698342627544No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14530.16651653819795412No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14460.1657143250063604No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14300.16388069485414616No Hit
GTTCAGTGGCAGTGGATCTGGGACACAGTTTACTTTCACCATCAGCAGCC14210.16284927789352568No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13890.15918201758909725No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG13790.15803599874396335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13700.15700458178334287No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13660.1565461742452893No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13640.1563169704762625No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGGAAC13480.15448334032404828No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGCCAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA13290.1523059045182939No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13020.1492116536364324No Hit
TTATCATACCGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTGTGTC12950.14840944044483867No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12950.14840944044483867No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGGAAGATGGAAGTGAGACATACTA12510.14336695752624956No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12480.1430231518727094No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12370.14176253114306211No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12350.14153332737403534No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12030.13786606706960688No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11870.13603243691739267No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11860.1359178350328793No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT11760.1347718161877454No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC11750.134657214303232No Hit
GTCCACATAGTATGTCTCACTTCCATCTTCCTTTATGTTGGCCACCCACT11690.13396960299615165No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11680.13385500111163828No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC11620.13316738980455795No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11540.13225057472845084No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTGTGTCCCAGATCCACTGCCA11400.1306461483452634No Hit
GGGTTCCCTGGCCCCAGGAGTCAAGGGGACCAGTCAAAATATCGTAATGCCCAAACTCTCTCGCACAGTAATATA11390.13053154646075002No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11370.13030234269172322No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11300.12950012950012948No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11230.12869791630853578No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11190.1282395087704822No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11010.12617667484924122No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10770.1234262296209199No Hit
ACATATTACTGTCAACGGTATGATAATCTCCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10590.12136339569967888No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT10500.12033197873905839No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10470.11998817308551822No Hit
ATATTACTGTCAACGGTATGATAATCTCCCTCCCACTTTTGGCCAGGGGA10460.11987357120100484No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10300.11803994104879059No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10150.11632091278108976No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC10020.11483108828241571No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9960.11414347697533539No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG9770.111966041169581No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTGTGTCCCAGATCCACTGCCA9730.11150763363152745No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9700.11116382797798727No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA9700.11116382797798727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9590.10990320724833999No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9510.10898639217223288No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT9320.1068089563664785No Hit
GTGTGAGGTGCAACTGGTGGAGTCTGGGGGAGGCTTGGTCCGGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC9210.1055483356368312No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9180.10520452998329104No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9160.10497532621426427No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9140.10474612244523748No Hit
CAGTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACGGTATGATAATCTC9130.1046315205607241No Hit
GTCTGCATCTGTTGGAGCCAGAGTCACCATCACTTGCCAGGCGAGTCAGG9100.10428771490718393No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9080.10405851113815716No Hit
ACATAAAGGAAGATGGAAGTGAGACATACTATGTGGACTCTGTGAAGGGCCGATTCATCATCTCCAGAGACAACG9080.10405851113815716No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9030.1034855017155902No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC8930.10233948287045633No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT8890.10188107533240277No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC8840.1013080659098358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA200.00734185551.7795371
GTGGTAT2750.047.6999361
GTATCAA12400.038.9738461
TAGTTGC400.00247136534.84780554
TATAGGG501.8617144E-434.4979062
TCTGGAG13200.034.0544870
TATCAAC14350.033.6564942
ATCAACG14850.032.4971733
TCAACGC15050.032.0542954
CAACGCA15550.030.8037745
AACGCAG16000.030.1527986
AGAGTAC17350.027.81139611
TCAGTAC500.007766573727.5761723
CGCAGAG17700.027.4514588
GTCATTC550.00840173927.13514768
AGCGCTG4350.026.9345175
CAGCGCT4350.026.9329744
CAGAGTA17950.026.87868510
GCAGAGT18550.026.1935739
TTCGGGG10100.026.06829870
Produced by FastQC (version 0.11.5)