FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027637
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38210.37182390279836164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31150.3031226006848722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30720.29893824375728006No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26290.25582963634045874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24670.2400653148923209No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22660.22050587902148325No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22430.21826773461835258No Hit
GGCTAACAGTTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22290.21690538585122957No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21530.20950977825827602No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21120.20552004258313003No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC20710.20153030690798404No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT20250.1970540181017227No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19970.19432932056747665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19770.1923831080430152No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19710.19179924428567677No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18330.1783703778668927No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAA17720.17243442966728523No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17440.1697097321330392No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17140.166790413346347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17080.16620654958900857No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC16940.16484420082188556No Hit
GATCTATGGTGCAGCCACTTTGCAACTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA16770.1631899201760933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16530.16085446514673957No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16500.16056253326807032No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16420.15978404825828574No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15720.15297230442267065No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15420.15005298563597846No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15020.14616056058705554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14990.14586862870838632No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14880.14479821181993252No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14660.14265737804302492No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14640.14246275679057877No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13930.13555370232874062No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12920.12572532908021022No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12680.12338987405085648No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12650.12309794217218725No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12570.12231945716240267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTCGAGGGAAA11830.11511847082189529No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11780.11463191769077992No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACTTT11640.1132695689236569No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11640.1132695689236569No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTC11360.11054487138941085No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11160.10859865886494939No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10760.10470623381602648No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10620.10334388504890345No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10520.10237077878667272No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10410.10130036189821892No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10350.10071649814088048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT1151.8189894E-1255.7060970
TAAGCGC200.00718598352.0619747
GTATCAA20600.040.9393731
GTGGTAT2350.039.5492441
CGTATGC557.2370476E-637.75393741
TCGTATG709.533014E-734.5925440
GTAATAC400.002626879434.4174653
TATCAAC24450.034.3470842
ATCAACG25400.033.333453
TCAACGC25500.033.2027324
CAACGCA25900.032.689955
TGTTAGA8050.032.62785370
AACGCAG25900.032.5570646
TTCGGGG4450.031.671170
CGTAAGG450.004676940430.5933023
TAGTTAG550.004963747730.2226769
CGCAGAG28350.029.7405938
AGAGTAC28250.029.60223211
GCAGAGT29300.028.7763089
AGTACGG27250.028.03781913
Produced by FastQC (version 0.11.5)