FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030371
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23520.22826729401351553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17590.17071520840551607No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAAATA16180.15703081705521604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15890.15421629684841673No Hit
GTATTATTGTGTGAGAGAGATTCATGGGTATGATTACGTTTGGGAAAACTATCGTTATAGGAGCTACTACTTTGA15840.15373103474379618No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCCCCTGGCCGAACGTC14630.1419876918119784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14330.139076119184255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14090.13674686108207626No Hit
CTCCTGATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTT14050.1363586513983798No Hit
ACTTATTACTGTCTACAAGATTACAATTTCCCTCGGACGTTCGGCCAGGGGACGAAGGTGGAAATCAAACGAA13890.13480581266359398No Hit
GATCTATGGTGCCTCCAAATTACAAGGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA13790.13383528845435286No Hit
GTAATAAGTTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG13750.1334470787706564No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13130.12742982867336136No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13080.12694456656874076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12750.12374183667824502No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12650.12277131246900387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12620.12248015520623154No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12220.11859805836926698No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGATATGATTTAAGCTGGTATCAGCAGAAA11950.11597764300431593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11940.1158805905833918No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11450.11112502195811022No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11370.11034860259071733No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTAGCAACTTATTACTGTCTACAAGATTACAATTTC10430.10122567502385063No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCC10370.10064336049830594No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10350.10044925565645772No Hit
GTCTACAAGATTACAATTTCCCTCGGACGTTCGGCCAGGGGACGAAGGTGGAAATCAAACGAACTGTGGCTGCAC10340.10035220323553361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.039.0203671
TATCAAC23750.033.777282
TCAACGC23850.033.6372874
ATCAACG23850.033.6356543
CAACGCA24750.032.4141125
AACGCAG25150.032.0339286
CTAGCGC1400.032.00298728
ACTAGCG1501.8189894E-1229.865127
CGCAGAG27350.029.5830448
AGAGTAC27600.029.31366211
CAGAGTA28000.028.77333310
GTCGTAC605.482305E-428.6955381
AATAGCG1352.419256E-1028.68297462
GCAGAGT29150.027.7563049
ACGCAGA29050.027.733337
TAGCGCT1503.45608E-1127.57716229
CGTATGC1607.4578566E-1125.9963341
CTATCGT5600.025.50572649
GTGGTAT3250.025.428661
AGTACGG22350.025.10853213
Produced by FastQC (version 0.11.5)