FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842781
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA20680.24537809941135358No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACACTCAGG19030.22580005956470303No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGCTATCCCATGTT18870.2239015829735127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17390.2063406745050019No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15410.18284702668902122No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14920.17703294212850076No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14860.1763210134068044No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13840.16421822513796586No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13260.1573362474949008No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12460.14784386453894902No Hit
GTGTATTACTGTGCGAGAGATGAGTTGATTTTTGGAGTGTTCGGGTACGG12340.14642000709555625No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12110.14369094699572013No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12080.14333498263487193No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11750.13941937466554183No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11050.131113539579084No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10590.12565541937941171No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10400.12340097842737317No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10090.11972268003194186No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9970.1182988225885491No Hit
CCCAAACACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC9970.1182988225885491No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG9950.1180615130146503No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAG9940.11794285822770091No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA9090.10785720133700213No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG9090.10785720133700213No Hit
CGTCCATACCGTACCCGAACACTCCAAAAATCAACTCATCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCT8930.10595872474581179No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTTTTCCCC8840.1048908316632672No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8430.10002598539834193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA200.00725166951.941538
GGTATCA6650.043.1630441
TATCGCC350.00127683639.863660
CTATCGC350.001279846639.84451359
GTATCAA15900.039.367431
TGGTATC2100.034.5721742
AATACCC400.002570971834.5680735
TATCAAC18200.034.192262
TCAACGC18650.033.733994
ATCAACG18650.033.550633
TCCGAGA6450.033.12184570
GTGGTAT2100.032.9356541
CAACGCA19050.032.8442045
AACGCAG19650.031.8413286
TATATAG450.004574988530.730822
CGCAGAG20900.029.928068
AGAGTAC20600.029.86063811
CAGAGTA21000.029.29186410
TGTGTCG7100.029.20457570
TATTAGA1352.9831426E-1028.169922
Produced by FastQC (version 0.11.5)