FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842781
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19700.23374993029031269No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA18360.21785018883909343No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17950.21298534257416815No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16620.19720425590989832No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG15850.1880678373147947No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGCTATCCCATGTT15450.18332164583681881No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC14640.17371060809391764No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACACTCAGG14060.1668286304508526No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13780.16350629641626946No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12500.1483184836867466No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12240.1452334592260623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11610.13775820764825025No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11070.1313508491529828No Hit
GTGTATTACTGTGCGAGAGATGAGTTGATTTTTGGAGTGTTCGGGTACGG10890.12921506298789365No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10830.12850313426619728No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10490.12446887150991777No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10260.12173981141008162No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10240.12150250183618283No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10060.11936671567109368No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9240.1096370231412431No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8920.10584006995886239No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8850.10500948645021661No Hit
CGTCCATACCGTACCCGAACACTCCAAAAATCAACTCATCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCT8720.10346697421987443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.043.426021
GTATCAA16500.043.3821561
GGTATCA5850.042.5599751
TATCAAC19350.036.606832
TCAACGC19500.036.1094364
ATCAACG19700.035.751333
CAACGCA20000.035.2087945
TGGTATC2450.033.848112
AACGCAG21100.033.700456
CGCAGAG22350.031.506758
AGAGTAC22450.031.36640711
CAGAGTA23100.030.33437210
GCAGAGT23400.030.0929859
TCCGAGA4950.028.34363770
GGATGGG9500.028.19446270
GTAGTTT2053.954119E-827.99798470
TTAACGG1254.0581654E-927.65740835
TTTAACG1306.179107E-926.58734134
ACGCAGA26900.026.177547
CGCGGTA1607.2759576E-1126.00919543
Produced by FastQC (version 0.11.5)