FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546517
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32830.21228347312056708No Hit
GTGCATCCACTGTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGA27240.17613773401779612No Hit
GTGGATGCACCATAAATCAGGAGCTGAGGAACTTTCCCTGGTTTGTGCTGATACCAGGCTAAATAATTCCTAATG22610.14619949214913253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22530.14568220071295693No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20700.1338491591104398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18180.11755447887090797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17020.11005375304636159No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTGCTCTGG16590.10727331157691768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16590.10727331157691768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.10701466585882988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15730.10171242863802984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15710.10158310577898595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44350.040.485951
TATCAAC50850.035.4382822
ATCAACG51850.034.7548073
TCAACGC51850.034.7548074
CAACGCA52550.034.2918475
AACGCAG53400.033.7460066
CGTATGC1350.033.36193541
GTGGTAT6250.032.5336071
ACGCAGA59150.030.4625827
CGCAGAG59300.030.4436268
CGACGTC1700.030.4267430
AGAGTAC59200.030.32046311
GACTCGA1950.030.05099526
CAGAGTA60850.029.61153410
CTCGACG1750.029.5516728
GGTATCA28650.029.2308791
GCAGAGT62950.028.5689689
TCGTATG1653.6379788E-1227.27484340
AGTACGG49450.026.1267113
GAGTACG50250.025.77932512
Produced by FastQC (version 0.11.5)