FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580165
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50490.3195235940550512No Hit
ATATTATAGTGATCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT48980.3099676299626938No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTTATGGTGAGAGTGAAATCTGTC44780.2833881271892492No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37230.235608306727462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32500.20567472384213042No Hit
CACCATAAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGC31070.19662503599307668No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30970.19599219068894705No Hit
CTCCTAACCTACTCATTTACTGGTCATCTACCCGGGAATCCGGGGTCCCT29680.1878284862656748No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29180.18466425974502662No Hit
GTTTTATCCAGCTCCCACAATAAGAACTACTTAGCTTGGTACCAACAGAA28680.18150003322437847No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28560.1807406188594229No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27810.17599427907845067No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCAGGTGC25500.16137555255305616No Hit
GTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATC25320.16023643100562282No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24120.15264228735606725No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23830.1508070359740913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23460.14846550834881167No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23230.14700996414931353No Hit
GGTTAGGAGGCTGTCCGGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTGGGAGCTGGATAAAACACT22740.14390902215907833No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22040.1394791050301709No Hit
GTAATAGACGGCCGTGTCTGCGGCAGACACAGACCTCAGACTCAGGGAGA21200.134163204475482No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATAAGCAGCC19420.12289855806197454No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19370.12258213540990973No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19000.12024060778463008No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18810.11903820170678378No Hit
CTCCCTGCCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATCCAGCTCCCA18300.11581069065572265No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17780.11251989507424859No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17740.11226675695259672No Hit
GCCTCCTAACCTACTCATTTACTGGTCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT17520.11087449728351152No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17440.11036822104020783No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17430.11030493650979487No Hit
CAATAAGAACTACTTAGCTTGGTACCAACAGAAACCCGGACAGCCTCCTAACCTACTCATTTACTGGTCATCTAC17250.10916581496236152No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17080.10808997794534116No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16190.10245765473858742No Hit
GATTTCACTCTCACCATAAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTGAT16060.10163495584321891No Hit
GGATCTCAGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGCCTGTGTCTCTGGGCGAGAGGG16010.10131853319115408No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15920.10074897241743742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.042.605791
GTATCAA28250.038.7471431
TATCAAC32450.033.5118562
ATCAACG34300.031.9056633
TCAACGC34450.031.4661124
CAACGCA34800.031.0524045
AACGCAG35400.030.527066
CAGTTCG23150.028.1044770
GTGGTAT4800.028.055771
ACGCAGA39950.027.1341067
CGCAGAG40050.026.8077588
AGAGTAC39850.026.7690411
TGGTATC5050.026.6609652
CAGAGTA40500.026.42465210
AAGTGAA10300.025.50968770
GCAGAGT42600.025.122039
TGTTAGA7750.025.02378770
AGTACGG38200.022.77186413
GAGTACG38900.022.36279712
GTAGTTT2803.9228325E-822.34266770
Produced by FastQC (version 0.11.5)