FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303785
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31850.039.0178151
CTCGTAT1550.033.4963339
GGTATCA14650.032.661971
TCAACGC38750.032.3254744
ATCAACG39450.031.9263533
CAACGCA39900.031.4800365
TATCAAC40200.031.3331172
AACGCAG40950.030.8409256
GTGGTAT6300.029.5065461
TTACGTA605.2139064E-428.9434344
CGTATGC1700.028.54105241
ACGCAGA44700.028.32957
CGCAGAG45200.028.016128
AGAGTAC44700.027.79062311
CAGAGTA45900.027.13904210
GCAGAGT48750.025.9053849
TCGTATG2100.024.73874940
GAGTACG39100.024.7302312
AGTACGG39750.024.41240513
CGATTCG902.1640517E-422.9407339
Produced by FastQC (version 0.11.5)