FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558016
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCTCGGCGCTCAGGCTGTTCATCTGGAGATACA16020.28708854226402114No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14700.26343330657185454No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10570.18942109186833353No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG10540.18888347287532972No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10000.1792063310012616No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9840.17633902970524143No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9680.17347172840922123No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9400.16845395114118591No Hit
CAGTAATACACGGCCGCGTCCTCGGCGCTCAGGCTGTTCATCTGGAGATA9400.16845395114118591No Hit
GTGTATTACTGTGTGAGAGCCCTCCATTACGTTGCCCATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTA9120.1634361738731506No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8790.15752236495010896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8680.15555109530909508No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8610.15429665099208625No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8430.15107093703406355No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8360.14981649271705472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8330.1492788737240509No Hit
GGATATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT8220.14730760408303703No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8040.14408189012501432No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7830.14031855717398783No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7600.1361968115609588No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG7510.13458395458194747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7470.13386712925794242No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGG7470.13386712925794242No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7460.13368792292694115No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG7340.13153744695492603No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7280.13046220896891844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7140.1279533203349008No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAAAAGTACAACAGTTAT7050.12634046335588944No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7020.12580284436288566No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC6920.12401078105287304No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC6890.12347316205986925No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGGATATTAGTACTTGGTTGGCCTGGTATCAGCAGAAA6800.12186030508085789No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6790.12168109874985665No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6680.11970982910884276No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGACGTGAAACTGGTGGAGT6670.1195306227778415No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6650.11917221011583896No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6470.11594649615781626No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6280.11254157586879229No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6250.11200395687578851No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6200.1111079252207822No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT6030.10806141759376076No Hit
ACGTAATGGAGGGCTCTCACACAGTAATACACGGCCGCGTCCTCGGCGCT6030.10806141759376076No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5950.10662776694575066No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5930.10626935428374813No Hit
GAGTAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATGGGCAACGTA5660.10143078334671407No Hit
CTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGGCTGGAGTGGG5640.10107237068471153No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG5600.10035554536070651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1150.042.0755961
GTATCAA7200.039.842421
TATCAAC9050.031.3159332
ATCAACG9100.031.143873
TCAACGC9000.031.1058864
CAACGCA9200.030.4296725
AACGCAG9700.028.861136
CGTTGTC6500.027.9299863
GTATTAG500.00766401427.6496771
CAGCGCT1900.027.2858684
GCGTTGT6800.026.67845362
ACTCTGA1158.554204E-426.58549770
AGAGTAC10850.026.43920911
GGCGTTG7000.025.90684161
CGCAGAG11000.025.4502728
CAGAGTA11250.025.19192910
ACGCAGA11200.024.9958027
TTGTCTC7150.024.99082465
AGCGCTG1801.2732926E-1124.9615155
GTTGTCT7200.024.7677864
Produced by FastQC (version 0.11.5)