FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979775
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23040.2351560307213391No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21330.21770304406623972No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATCTGCAGATACA20560.2098440968589727No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18230.18606312673828174No Hit
GTGTATTACTGTGCGAGAGGTGGCAATTTGGGGTGGTGGCTGATTCGGGAACGATACCAGTACTACTTTGACTA18170.1854507412416116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16440.16779362608762216No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15520.1584037151386798No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15040.15350463116531857No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12810.13074430353907784No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12740.13002985379296267No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12680.1294174682962925No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12660.12921333979740246No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCAGCCAATAAGCAC12510.12768237605572708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12190.12441632007348626No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12190.12441632007348626No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12080.12329361332959098No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11960.12206884233625066No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11870.12115026409124544No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11470.1170676941134444No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11160.11390370238064862No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11120.11349544538286852No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11100.11329131688397848No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10900.11125003189507796No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10160.10369727743614605No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10130.10339108468781098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9950.10155392819780051No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9910.10114567120002042No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT9900.10104360695057538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG150.0023721968.876825
GTATCAA19450.042.22681
ATCAACG23350.035.1236233
TATCAAC23850.034.2743072
TCAACGC24000.034.0079354
CCGCTAT1301.1128559E-633.9094870
CAACGCA24150.033.7967035
AACGCAG24250.033.6573336
AGAGTAC26150.031.08017311
CGCAGAG27100.030.3718838
GCAGAGT27400.030.0378139
GTGGTAT4300.029.6936821
GTTAGCC703.881404E-529.536733
CAGAGTA27900.029.13070110
ACGCAGA29550.027.853747
ACTAGCT500.007505409427.77045444
ATAGCCG500.007794325727.55635616
GTACGAC500.007802096727.55072811
AGTACGG26300.025.40583213
GAGTACG26600.024.98854412
Produced by FastQC (version 0.11.5)