FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748677
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8620.11513643400291448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.042.6218571
GTATCAA26200.040.74781
TCAACGC30800.034.5926324
ATCAACG30600.034.500213
GTGGTAT3700.034.438461
TATCAAC30800.034.4131282
GCCGCGT400.002644464434.3694538
CAACGCA31400.033.7127155
AACGCAG31650.033.446426
ACGCAGA33450.031.6466127
CGCAGAG33600.031.3007558
TGGTATC4150.030.6815832
AGAGTAC34950.030.58545111
CAGAGTA35650.029.98289110
GCAGAGT36950.028.741989
CGTGATA605.5268506E-428.65461222
CGGGTAA500.00785708627.51026517
TACGGGT6000.027.50658815
AGTACGG27700.027.3061613
GAGTACG27850.027.27889412
Produced by FastQC (version 0.11.5)