FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584273
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13490.23088521975172566No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11520.19716810463601778No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT11090.18980853128588862No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10460.1790259005636064No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10230.17508938458563036No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9550.16345098952030987No Hit
CCCTTAGACTCTCCTGTGAAGCCTCTGGATTCAGTTTCAGTGACGCCTGG9130.15626256903878838No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8750.1497587600316975No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8640.1478760784770133No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8510.1456510911851138No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8060.13794921209776936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830.13401269611979333No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT7380.12631081703244887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7170.12271660679168814No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7100.12151853671143456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7090.1213473838428269No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6950.11895124368231974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6940.11878009081371207No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6840.11706856212763553No Hit
GATCTACGCTGTAGCCAGTTTGCACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA6820.11672625639042022No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6790.11621279778459725No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6730.11518588057295134No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6680.11433011622991307No Hit
GCCCCTAAGGTCCTGATCTACGCTGTAGCCAGTTTGCACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGAT6670.1141589633613054No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6630.1134743518868748No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6570.11244743467522886No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6560.11227628180662122No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6500.11124936459497528No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6500.11124936459497528No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6480.11090705885775998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6450.11039360025193702No Hit
CCTTAGGGGCTGTCCCTGGTTTTTGATAATACCAATTTAAATAGGTGCTA6210.10628593140535332No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6200.10611477853674567No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6170.10560131993092271No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG5900.10098019247851604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG200.007455565551.5765045
GTGGTAT1700.046.5957531
GTATCAA10050.042.150861
GGTATCA3800.037.1593131
TATCAAC11950.035.1337432
GTCTAAA400.00246796334.8556451
TGGTATC2300.034.4137882
ATCAACG12950.032.3985143
TCAACGC13300.031.5405244
AACGCAG13700.030.8706116
CAACGCA13600.030.8447745
ATCTAGG450.004660132330.6136321
CGCAGAG14750.028.673048
GCAGAGT14850.028.2484139
CTTCCGT500.007768109427.57354429
TCAGTCG500.007797423327.55227325
AATAGCG657.70883E-427.0679962
ACGCAGA16100.026.482357
CAGAGTA16150.026.18969310
TGTTAGA3650.025.94931270
Produced by FastQC (version 0.11.5)